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Home > Veterinary tests > Scanelis PCR Tests > Dogs > Leishmaniasis

Quantitative detection of pathogenic leishmania in dog (L. infantum, L. donovani, L. chagasi).

Reasons for testing

- Diagnostics of leishmaniasis in all forms, even chronic forms.
- Status determination of a heathy animal
- Treatment monitoring

Test characteristics

- Real-time PCR
- Limit of detection
- Quantitative analysis (on request, depending on the analyzed samples).

Diagnostic samples

- Clinical suspicion – depending on clinical signs:
blood (EDTA) and/or cutaneous scraping and/or lymph node aspirate (lymph node hypertophy) and/or conjunctival cells. We advise you to collect several samples in order to perform a pool analysis.
- Healthy animal: bone marrow (EDTA) preferably
- Treatment monitoring: blood (EDTA) (blood is the earliest sample that become negative) then bone marrow if blood is negative

Result interpretation

- Negative result: absence of leishmania or quantity lower than the limit of detection. Leishmaniasis can be excluded if the analyzed samples were appropriate.
- Positive result: presence of leishmania, compatible with leishmaniasis or a healthy carrier in an endemic area. The rate of asymptomatic carriers in blood was assessed with the Scanelis assay: 6%. If a cutaneous sample was collected, the detected load must be taken into account to interpret the result (contact us for interpretation of results).

Special case of treatment monitoring : monitoring can be performed on blood which is the first to become negative (followed by bone marrow). After a negative result on blood, bone marrow is the most adapted sample for monitoring.
The animal can be regarded as negative after 2 negative results on bone marrow (the second must be performed 3 months later).