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Canine Parvovirosis

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Quantitative detection (real-time PCR) of canine parvovirus for a reliable diagnostics of canine parvovirosis even if the animal has recently been vaccinated with a high titer attenuated vaccine.

Reasons for testing

- Diagnostics of canine parvovirosis
- Assessment of the epidemiological risk (to reveal viral spread in a group)
- Assessment of environmental contamination and sanitizing monitoring in an infected environment

Test characteristics

- Real-time PCR
- Limit of detection
- Quantitative analysis
- Possible strain typing on request (this typing is not necessary for routine diagnostics)

Diagnostic samples

- Rectal swab (even post-mortem, dry swab without any preservative or culture media)
- Environmental swab

Result interpretation

Most of young dogs are infected with parvovirus. The only presence of virus, either wild or vaccine strain, does not lead to a reliable diagnostics if the viral load shed is not quantified. The quantitative results must be interpreted in a clinical and epidemiological context.

  • Positive result :
    • Low or very low viral load : healthy carrier or a low load of vaccine strain is detected in the analyzed sample. Then, parvovirosis can be excluded.
    • High or very high viral load : compatible with parvovirosis. Such loads can be detected in some rare asymptomatic animals in a very contaminated environment (presence of clinical cases)
    • Medium viral load : eventually compatible with a recent vaccination with a high titer vaccine. Medium viral load are most often detected in cases of coinfections with coronavirus or in subacute forms of parvovirosis (duration of clinical signs > 10 days).
  • Negative result : absence of virus or quantity lower than the limit of detection of the assay.